Phenotypic and Genotypic Characterization of Salmonella Typhi isolated from Human Blood with Distribution of Virulence Genes and Sequencing

A cross-sectional study was conducted in the Department of Microbiology at tertiary care hospital Bangladesh from July 2019 to June 2020, see phenotypic and genotypic characterization Salmonella Typhi isolated human blood with distribution virulence genes sequencing. Clinically suspected enteric fever patients irrespective age gender who attended either or Medicine out-patient department (MOPD) a level for culture sensitivity test were included this study. The positive growth S. identified by gram straining, colony morphology biochemical test. Then using specific antisera. Final identification done 16s Rrna PCR. Virulence factors like hemolysin siderophore detected siderophores production assay. PCR sequencing done. total number 50 organisms as species which 40 (48.2%) isolates. Among Typhi, no produced 30 (75.0%) isolates have ability produce siderophore. all (100%) viaB staA gene, 21 (52.5%) iroB. Sequencing iroB further validation. In conclusion carried staA, responsible pathogenicity.
 CBMJ 2023 January: Vol. 12 No. 01 P: 41-47